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KMID : 0380020040190010072
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Korean Journal of Biotechnology and Bioengineering
2004 Volume.19 No. 1 p.72 ~ p.77
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16S rDNA-PCR and RFLP Analysis for rapid Identification of Spoilage Bacteria from low Salt Cucumber Brine
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Kim Jae-Ho
Jang Hye-Young
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Abstract
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The aim of this study was to isolate and identify the spoilage bacteria in the low salt cucumber brine. The PCR amplicons comprising a portion of the 16S rRNA gene of the isolated colonies were directly sequenced and the untrimmed whole sequencing results of the unknown strains were aligned with the type strains using BLAST of NCBI. Then Sequence Aligner and Sequence Match of RDP confirmed the outcome. The identified isolates were eight species and belong to three genuses: Clostridium, Lactobacillus, and Bacillus. The RFLP pattern of the 16S rRNA gene of isolates verified the identified species. From now on the complex spoiling process of law salt fermented cucumber could be analyzed using the isolated species individually or with certain combinations.
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KEYWORD
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Spoilage bacteria, low salt cucumber brine, 16s rDNA-PCR
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